![]() ![]() To open this, click the compensation node UNDER the “Compensation” group.ĭifferent matrices can be distinguished by color and by name. Matrix name is derived from the compensation interface while creating the matrix and matrix color is derived from the same location. 2.3.6 Manual compensation using the 8×8 compensation matrix. When fcs3.0 files are loaded into FlowJo, the acquisition matrix is applied automatically using the $SPILL keyword embedded within the file. FLOWJO 10 MANUALLY ADD COMPENSATION MANUAL Compensation controls are required for each fluorochrome and should contain both a positive and a negative population. This MACSQuantify Software guide applies to the MACSQuant Analyzer 10 ( 130-096-343), the. FLOWJO 10 MANUALLY ADD COMPENSATION SOFTWARE These controls should be solely used to set compensation. The positive should be at least as bright as anything that will be encountered in the experiment and should form at least 10 of the population. The acquisition matrix can be modified by double clicking on any compensation matrix badge next to a sample. ![]() ![]() Click edit to modify the values, give the matrix a new name and use the to apply it to any sample. If you want to reset it, simply open the matrix editor again and click reset.Ĭompensation matrices never need to be removed!įirst off, you always have access to the uncompensated parameters in the graph window (graph > show uncompensated parameters). Load your single-stained controls into FlowJo, and make sure they are all added to FlowJo’s Compensation group. Secondly, you can always create a new matrix in FlowJo and overwrite the current matrix. Thirdly, all your matrices created in the workspace are present in the matrix editor. FLOWJO 10 MANUALLY ADD COMPENSATION SOFTWARE.FLOWJO 10 MANUALLY ADD COMPENSATION MANUAL. ![]()
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